Journal: Nature
Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
doi: 10.1038/s41586-025-08915-1
Figure Lengend Snippet: a , Mouse AML cells treated with 50 nM GSK–LSD1 and different concentrations of LY2090314 for 5 days; cell growth was determined by the Cell-Titer-Glo assay. Data are presented as mean ± s.d. from two biological independent experiments. BMDM, bone marrow-derived macrophage; RLU, relative light unit. b , Quantification of colonies formed by normal mouse LSK cells treated with the indicated inhibitors. Data are presented as mean ± s.d. from three biological independent experiments. P values were determined using two-way ANOVA. NS, not significant. c , Flow-cytometric quantification of CD11b and Gr-1 expression in the cells harvested from methylcellulose in panel b . d , Analysis of cell viability in THP-1 cells treated with the indicated inhibitors for 5 days by the Cell-Titer-Glo assay. Data are presented as mean ± s.d. from three biological independent experiments. P values were determined using two-way ANOVA. e , Analysis of CD11b mRNA relative level in THP-1 cells treated with the indicated inhibitors for 5 days. Values were normalized against GAPDH . Data are presented as mean ± s.d. from three biological independent experiments. P values were determined using two-way ANOVA. f , Quantification of colonies formed by THP-1 cells treated with the indicated inhibitors. Data are presented as mean ± s.d. from three biological independent experiments. P values were determined using two-way ANOVA. g , h , Tumour burden ( g ) and Kaplan–Meier survival curves ( h ) of mice treated with vehicle ( n = 15), GSK–LSD1 ( n = 9), LY2090314 ( n = 11) or a combination of both inhibitors ( n = 20) in a syngeneic model of a HOXA9–MEIS1-driven AML model. P values were determined using the log-rank test. The error bars represent mean ± s.e.m. ( g ). P values were determined using two-way ANOVA.
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).
Techniques: Glo Assay, Derivative Assay, Expressing
